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KMID : 0811720020060020093
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Korean Journal of Physiology & Pharmacology
2002 Volume.6 No. 2 p.93 ~ p.100
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Increase of Intracellular Ca2+ Concentration Induced by Lysophosphatidylcholine in Murine Aortic Endothelial Cells
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Ki Whan Kim/Mei Hong Zhu
Sung Jin Park/Hyun Jin Kim/Dong Ki Yang/Suk Hyo Suh*/In Suk So/Ki Whan Kim
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Abstract
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Effects of oxidized low-density lipoprotein (ox-LDL), 1?α?stearoyl?lysophosphatidylcholine (LPC), on intracellular Ca2+ concentration were examined in mouse endothelial cells by measuring intracellular Ca2+ concentration ([Ca2+]i) with fura 2-AM and reverse transcription-polymerase chain reaction (RT-PCR). LPC increased [Ca2+]i under the condition of 1.5 mM [Ca2+]o but did not show any effect under the nominally Ca2+?free condition. Even after the store depletion with 30μM 2,5-di-tert- butylhydroquinone (BHQ) or 30μM ATP, LPC could still increase the [Ca2+]i under the condition of 1.5 mM [Ca2+]o. The time required to increase [Ca2+]i (about 1 minute) was longer than that for ATP-induced [Ca2+]i increase (10∼30seconds). LPC-induced [Ca2+]i increase was completely blocked by 1μMLa3+. Transient receptor potential channel(trpc) 4 mRNA was detected with RT-PCR. From these results, we suggest that LPC increased [Ca2+]i via the increase of Ca2+ influx through the Ca2+ routes which exist in the plasma membrane.
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